Circumventing qPCR inhibition to amplify miRNAs in plasma
نویسندگان
چکیده
منابع مشابه
SPUD qPCR Assay and PREXCEL-Q 129 SPUD qPCR Assay Confirms PREXCEL-Q Software’s Ability to Avoid qPCR Inhibition
Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. When removal of inhibitory substances by column or reagent-based methods fails or is incomplete, t...
متن کاملSPUD qPCR assay confirms PREXCEL-Q softwares ability to avoid qPCR inhibition.
Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. When removal of inhibitory substances by column or reagent-based methods fails or is incomplete, t...
متن کاملEvaluation of extraction kits and RT-qPCR systems adapted to high-throughput platform for circulating miRNAs
MicroRNAs regulate gene expression at the post-transcriptional level. Differential expression of miRNAs can potentially be used as biomarkers for early diagnosis and prediction for outcomes. Failure in validation of miRNA profiles is often caused by variations in experimental parameters. In this study, the performance of five extraction kits and three RT-qPCR systems were evaluated using BioMar...
متن کاملMaternal Plasma miRNAs Expression in Preeclamptic Pregnancies
OBJECTIVE Preeclampsia (PE) is a pregnancy-specific syndrome and one of the leading causes of maternal and fetal morbidity and mortality. The pathophysiological mechanisms of PE remain poorly known. Recently, circulating miRNAs are considered as potential useful noninvasive biomarkers. The aim of this study was to identify differentially expressed plasma miRNAs in preeclamptic pregnancies compa...
متن کاملNo Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People
BACKGROUND MicroRNAs (miRNAs) are small noncoding RNAs that are involved in many biological regulation processes. Studies have reported that miRNAs are enriched in human plasma and plasma-derived exosome as novel diagnostic biomarkers. The aim of this study was to determine whether the miRNA expression levels are different between plasma and plasma-derived exosome. METHODS We sequenced and qu...
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ژورنال
عنوان ژورنال: Biomarker Research
سال: 2014
ISSN: 2050-7771
DOI: 10.1186/2050-7771-2-13